Inkingi-Sniper Inkingi Chromasir HPLC UPLC inkingi ikuraho impinga yimizimu

ibisobanuro bigufi:

Inkingi ya Ghost-Sniper nigikoresho gikomeye cyo gukuraho impinga yimizimu yakozwe mugihe cyo gutandukana kwa chromatografique, cyane cyane muburyo bwa gradient.Impinga yumuzimu izatera ibibazo byinshi iyo impinga yumuzimu irenze impinga yinyungu.Hamwe na Chromasir umuzimu-sniper inkingi, ibibazo byose byimpinga yumuzimu birashobora gukemuka kandi ikiguzi cyo gukoresha ikigeragezo gishobora kuba gito cyane.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Inkingi ya Ghost-sniper yakozwe muburyo bwo gukuraho impinga yimizimu.Impinga ya Ghost ifite inkomoko itazwi muri chromatogramu, mubisanzwe igaragara mugihe cyo gutandukana kwa chromatografique mugihe cyo gukuraho buhoro buhoro cyangwa gukora igihe kirekire.Kuba impanuka yimpanuka igira uruhare runini mubushakashatsi bwabasesenguzi.Inkingi ya Ghost-sniper ibaho kugirango ikemure ibyo bibazo kandi itume inzira yo kugerageza yoroshye kandi neza.Iyi nkingi irashobora gukoreshwa muburyo bukabije kandi ikerekana ingaruka zikomeye zo gufata.Nukuri rwose ninzira nziza yo gukuraho intambamyi ziva kumuzimu kuburyo bwo kugenzura no gusesengura ibintu.

Ibipimo

Igice No.	Igipimo	Umubumbe	Gusaba
MC5046091P	50 × 4.6mm	hafi 800ul	HPLC
MC3546092P	35 × 4.6mm	hafi 580ul	HPLC
MC5021093P	50 × 2.1mm	hafi 170ul	UPLC
MC3040096P	30 × 4.0mm	hafi 380ul	HPLC Ingano yinkingi

Kwinjiza

Porogaramu n'ibisubizo

Kwirinda

1. Niba isesengura ryicyiciro ryateguwe muri sisitemu ya HPLC, birasabwa kongera igihe cyo kuringaniza igihe kingana niminota 5 - 10 min mubihe bya chromatografiya, kugirango bigaragaze ingano yubunini bwinkingi yizimu.
2. Ku nkingi nshya, shyiramo 100% acetonitrile ku kigero cya 0.5ml / min mu masaha 4 mbere yo kuyakoresha.
3. Ion-couple reagent mugice cya mobile, irashobora gutwarwa ninkingi-ya sniper inkingi, ishobora guhindura igihe cyo kugumana nuburyo bwo hejuru bwintego yawe.Nyamuneka koresha witonze munsi yicyiciro kigendanwa.
4. Inkingi yubuzima bwose iterwa nuburyo bwo gusesengura, nkicyiciro kigendanwa.isuku ya solvent, hamwe n'ibikoresho byanduye.Nyamuneka usimbuze umuzimu-sniper inkingi buri gihe kugirango umenye imikorere.
5. Gusimbuza umuzimu-sniper inkingi birasabwa niba gufata ingaruka birushijeho kuba bibi cyangwa ibyifuzo ntibishobora guhazwa.
6. Nkigice cyo kweza chromatografi yamazi, inkingi-ya sniper irashobora gushungura ibice bikomeye kandi ikuraho umwanda kama mbere yo gutera inshinge.Inkingi-ya sniper inkingi nayo itanga ibikoresho ninkingi ya chromatografi hamwe nuburinzi bwiza, kandi itunganya chromatogramu.
7. Niba icyiciro kigendanwa kirimo umunyu wa buffer, mbere na nyuma yo gukoresha, ohereza kumashanyarazi hamwe na 10% yumuti wicyiciro cya 10% (methanol cyangwa acetonitrile 10%), kugirango wirinde umunyu wa buffer hanze hanyuma uhagarike inkingi.
8. Nyamuneka menya ko impinga zose zuzimu zidashobora gufatwa ninkingi ya Ghost-Sniper.
9. Niba inkingi isize idakoreshejwe igihe kirekire, birasabwa kuyigumisha mumuti wamazi kama (70% methanol cyangwa acetonitrile).Nyamuneka nyamuneka uyisukure hamwe na acetonitrile 100% kumuvuduko wa 0.5 ml / min kumasaha 1 mbere yo kuyikoresha.