Inkingi-Sniper Inkingi Chromasir HPLC UPLC inkingi ikuraho impinga yimizimu

ibisobanuro bigufi:

Inkingi ya Ghost-Sniper nigikoresho gikomeye cyo gukuraho impinga yimizimu yakozwe mugihe cyo gutandukana kwa chromatografique, cyane cyane muburyo bwa gradient. Impinga yumuzimu izatera ibibazo byinshi iyo impinga yumuzimu irenze impinga yinyungu. Hamwe na Chromasir umuzimu-sniper inkingi, ibibazo byose byimpinga yumuzimu birashobora gukemuka kandi ikiguzi cyo gukoresha ikigeragezo gishobora kuba gito cyane.

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Ibicuruzwa birambuye

Ibicuruzwa

Inkingi ya Ghost-sniper yakozwe muburyo bwo gukuraho impinga yimizimu. Impinga ya Ghost ifite inkomoko itazwi muri chromatogramu, mubisanzwe igaragara mugihe cyo gutandukana kwa chromatografique mugihe cyo gukuraho buhoro buhoro cyangwa gukora igihe kirekire. Kuba impanuka yimpanuka igira uruhare runini mubushakashatsi bwabasesenguzi. Inkingi ya Ghost-sniper ibaho kugirango ikemure ibyo bibazo kandi itume inzira yo kugerageza yoroshye kandi neza. Iyi nkingi irashobora gukoreshwa muburyo bukabije kandi ikerekana ingaruka zikomeye zo gufata. Nukuri rwose ninzira nziza yo gukuraho kwivanga mumasonga yizimu muburyo bwo kugenzura no gusesengura ibintu.

Ibipimo

Igice No.	Igipimo	Umubumbe	Gusaba
MC5046091P	50 × 4.6mm	hafi 800ul	HPLC
MC3546092P	35 × 4.6mm	hafi 580ul	HPLC
MC5021093P	50 × 2.1mm	hafi 170ul	UPLC
MC3040096P	30 × 4.0mm	hafi 380ul	HPLC Ingano yinkingi

Kwinjiza

Porogaramu n'ibisubizo

Kwirinda

1. Niba isesengura ryicyiciro ryateguwe muri sisitemu ya HPLC, birasabwa kongera igihe cyongeweho mugihe kingana niminota 5 - 10 min mubihe bya chromatografiya yawe, kugirango bigaragaze ingano yubunini bwinkingi yizimu.
2. Ku nkingi nshya, shyiramo 100% acetonitrile ku gipimo cya 0.5ml / min mu masaha 4 mbere yo kuyakoresha.
3. Ion-couple reagents mugice cya mobile, irashobora gutwarwa ninkingi ya kizimu-sniper, ishobora guhindura igihe cyo kugumana nuburyo bwo hejuru bwintego yawe. Nyamuneka koresha witonze munsi yicyiciro kigendanwa.
4. Inkingi yubuzima bwose iterwa nuburyo bwo gusesengura, nkicyiciro kigendanwa. isuku ya solvent, hamwe n'ibikoresho byanduye. Nyamuneka usimbuze umuzimu-sniper inkingi buri gihe kugirango umenye imikorere.
5. Gusimbuza umuzimu-sniper inkingi birasabwa niba gufata ingaruka birushijeho kuba bibi cyangwa ibyifuzo ntibishobora guhazwa.
6. Nkigice cyo kweza chromatografi yamazi, inkingi yizimu-sniper irashobora gushungura ibice bikomeye kandi ikuraho imyanda ihumanya mbere yo gutera inshinge. Inkingi-ya sniper inkingi nayo itanga ibikoresho ninkingi ya chromatografi hamwe nuburinzi bwiza, kandi itunganya chromatogramu.
7.
8. Nyamuneka menya ko impinga zose zuzimu zidashobora gufatwa ninkingi ya Ghost-Sniper.
9. Niba inkingi isize idakoreshejwe igihe kirekire, birasabwa kuyigumisha mumuti wamazi kama (70% methanol cyangwa acetonitrile). Nyamuneka nyamuneka uyisukure hamwe na acetonitrile 100% kumuvuduko wa 0.5 ml / min kumasaha 1 mbere yo kuyikoresha.