Nigute ushobora guhitamo HPLC Isesengura no Kunoza Laboratoire

Muri laboratoire zisesenguye,Amazi meza ya Chromatografiya (HPLC)ni tekinike yingenzi yo gutandukanya, kumenya, no kugereranya ibice. Ariko, kugera kubisubizo bihamye kandi byizewe bisaba ibirenze ibikoresho byiza-bisabaGutezimbere. Iyi ngingo iragaragaza uburyo ushobora kuzamura ibyaweIsesengura rya HPLCkugirango arusheho gukora neza, kugabanya igihe cyateganijwe, no kunoza ukuri.

Inzitizi Zisanzwe Muri HPLC Isesengura nuburyo bwo kuzikemura

Mugihe HPLC nigikoresho gikomeye cyo gusesengura, ntabwo kirimo ibibazo. Ibibazo nkagukemura nabi, urusaku rwibanze, nibisubizo bidahuyeirashobora kubangamira imikorere ya laboratoire. Dore uko wakemura ibyo bibazo bisanzwe:

1. Icyemezo kibi

Kimwe mubibazo bikunze kugaragara muri HPLC ni ugutandukana nabi hagati yimpinga, akenshi biteweguhitamo inkingi itari yo cyangwa ibipimo bitembera neza. Kunoza imyanzuro:

• Hitamo aInkingihamwe n'ibikwiyeicyiciro gihagaze nubunini bukekubisesengura.

• Hinduraumuvuduko wo gutembera hamwe na gradient conditionskuzamura ubukana bwo hejuru no gutandukana.

• Koreshakugenzura ubushyuheguhagarika ibihe byo kugumana no kunoza imyororokere.

2. Gutwara ibanze cyangwa urusaku

Urusaku rwibanze rushobora kubangamira kumenya impinga no gutandukanya amakuru neza. Iki kibazo gikunze guterwa na:

•Imihindagurikire y'ubushyuhe- Komeza laboratoire ihamye kandi ukoreshe ifuru yinkingi nibiba ngombwa.

•Icyiciro kigendanwa cyanduye- Koresha umuyonga mwinshi kandi ushungure icyiciro cya mobile mbere yo gukoresha.

•Kwanduza ibikoresho- Buri gihe usukure kandi ubungabunge disiketi, pompe, na tubing kugirango ugabanye urusaku rwinyuma.

3. Kwishyira hamwe kw'impinga idahuye

Kwishyira hamwe bidahuye bigira ingaruka ku kwizerwa kwinshi. Kugira ngo iki kibazo gikemuke:

• Menya neza koInkingi ya HPLC itunganijwe nezambere yo gukoresha.

Komeza aumuvuduko uhamyekandi wirinde ihindagurika ryumuvuduko.

• Hindura nezaIgenamiterere rya porogaramu yo kwishyira hamwe, kwemeza ibisubizo bihoraho kandi byororoka.

Guhitamo Inkingi ya HPLC

Guhitamo neza inkingi ya HPLC niingenzi kugirango tugere ku gutandukana kwiza. Suzuma ibi bintu mugihe uhisemo inkingi:

•Uburebure bw'inkingi: Inkingi ndende zitanga gutandukana neza ariko kongera igihe cyo gusesengura. Hitamo uburebure buringaniza imiterere n'umuvuduko.

•Inkingi: Inkingi ngufi zitanga ibisubizo bihanitse ariko bisaba igitutu kinini. Wemeze guhuza na sisitemu ya HPLC.

•Icyiciro gihagaze: Hitamo icyiciro hamwe na chimie ikwiye kuri analyte yawe (urugero, C18 kubintu bitari polar, fenil kubintu bya aromatic).

Kunoza ibyiciro bya mobile hamwe nibiciro bitemba

Icyiciro kigendanwa ni urufunguzo rwo gusesengura HPLC. Dore uko wabitezimbere:

•Guhindura ibihimbano: Hindura nezaIkigereranyokunoza gutandukana. Koreshabuhoro buhoroKuri Ingero.

•Kugenzura urwego pH: Menya nezaicyiciro cya mobile pHni Byahujwe na Byombi Icyitegererezo na Inkingi.

•Hindura igipimo cyo gutemba: Igipimo kinini cyo kugabanya kigabanya igihe cyo gusesengura ariko gishobora kubangamira gukemura. Kuringaniza umuvuduko nibikorwa ukurikije uburyo bwawe.

Kubungabunga no Kwirinda

Kubungabunga neza biremezaimikorere ihamye kandi yongerera ibikoresho igihe cyose. Kurikiza ubu buryo bwiza:

•Isuku y'inzira: Buri gihe usukureinshinge, inkingi, na detectorkwirinda kwanduza.

•Simbuza Ibikoreshwa: HinduraIkidodo, Akayunguruzo, na tubingnkuko bikenewe kugirango wirinde kumeneka no guhindagurika k'umuvuduko.

•Hindura Sisitemu: Buri gihe uhindure disiketi nibindi bice byingenzi kugirango ubone ibisubizo nyabyo.

Umwanzuro

Gutezimbere isesengura rya HPLC ni ngombwa mugutezimbere imikorere ya laboratoire no kwemeza ibisubizo byiza. Mugukemura ibibazo bisanzwe nkagukemura nabi, urusaku rwibanze, hamwe no guhuza impinga bidahuye, no mu guhitamo iburyoinkingi n'ibice bigendanwa, urashobora kuzamura cyane imikorere yawe yisesengura. Ibisanzwekubungabunga no kwitondera uburyo bwizabizakomeza sisitemu ya HPLC ikora neza, igabanye igihe cyo hasi kandi urebe ibisubizo nyabyo, byororoka.

Kubuyobozi bwinzobere kuriGukoresha HPLC, umubonanoChromasir- dufite ubuhanga bwo gutangaIgisubizo cya chromatografiyagufasha laboratoire yawe kugera kurwego rwo hejuru rwimikorere.